Atila Real-Time PCR RhDGT Kit

The Rh blood group system is one of the most clinically significant blood group systems in transfusion medicine. The presence or absence of the Rhesus D (RhD) antigen on red blood cells (RBCs) determines an individual’s Rh status as either RhD positive or RhD negative. RhD-negative individuals may develop alloantibodies against RhD-positive RBCs after transfusion or pregnancy, which can lead to hemolytic disease of the fetus and newborn or complications in transfusion therapy.

The RhD gene is located on chromosome 1 and consists of multiple exons encoding the RhD protein. Deletions, insertions, and rearrangements in the RhD gene can result in RhD-negative phenotypes. One of the most common causes of RhD negativity is the complete deletion of the RhD gene, while some individuals carry an RhD pseudogene containing a characteristic 38-bp insertion in exon 4, which leads to a non-functional RhD protein.

The Atila Rhesus D Genotyping Testing (RhDGT) Kit is a molecular assay designed to detect the presence of RhD exon 4, exon 5, and exon 7, as well as the exon 4 pseudogene form with a 38-bp insertion. The kit utilizes real-time quantitative PCR (qPCR) to analyze genomic DNA or cell free DNA extracted from whole blood samples, providing a reliable and precise determination of RhD status at the molecular level and includes Internal Control (IC) for monitoring test performance in each individual test. This test is intended for research use only and should be performed by trained laboratory personnel with expertise in molecular biology techniques.