Atila HBV Assay Kit (Digital PCR)
The Atila HBV Assay Kit is a digital polymerase chain reaction (dPCR) test and is adapted for use on the QIAcuityTM system or the Bio-Rad QX200/QX600™ system. It is intended for the in vitro quantitative detection of human hepatitis B virus (HBV) DNA in human plasma or serum samples. This assay is designed for research use only and is not intended for use as a screening test for HBV infection or as an aid in the diagnosis, confirmation, or monitoring of HBV infection in patients.
The Atila HBV Assay Kit is intended for use by qualified and trained laboratory professionals who are experienced in nucleic acid amplification techniques and in vitro diagnostic procedures
This product is for research use only (RUO). Each kit includes 100 tests.
AHBV-100Q (QIAcuity™ Digital™ PCR System), AHBV-100B (Bio-Rad QX200/QX600 System)
Atila HBV Assay Kit (Digital PCR)
The genome of Hepatitis B virus (HBV) is a partially double-stranded circular DNA of about 3.2 kilobase (kb). HBV infection leads to a wide spectrum of liver disease ranging from acute hepatitis (including fulminant hepatic failure) to chronic hepatitis, cirrhosis, and hepatocellular carcinoma (HCC). Risk for chronic infection is related to age at infection: about 90% of infants with hepatitis B go on to develop chronic infection, whereas only 2%–6% of people who get hepatitis B as adults become chronically infected. HBV is spread when blood, semen, or other body fluids from a person infected with the virus enters the body of someone who is not infected. This can happen through sexual contact; sharing needles, syringes, or other drug-injection equipment; or from mother to baby at birth.
The Atila HBV Assay Kit comes in two formats, a droplet digital polymerase chain reaction (ddPCR) assay using Bio-Rad QX200/QX600™ system or a digital polymerase chain reaction (dPCR) assay using QIAcuity™ Digital™ PCR System. The assay is designed for absolute quantification of HBV DNA using Atila’s proprietary digital amplification technology. DNA oligos are designed to specifically detect HBV DNA. The assay does not discriminate against the HBV genotypes. The test also includes an Internal Control (IC) for monitoring test performance in each individual test.
The Atila HBV Assay is performed in three main steps, specimen processing and nucleic acid extraction; droplet digital PCR amplification; and data analysis and quantification. Specimen processing and nucleic acid extraction is performed with the Atila automated DNA/RNA extraction system, other compatible automated systems, or manual viral DNA/RNA extraction kits