Atila BioSystems Publications
At Atila Biosystems, we are at the forefront of revolutionizing diagnostic technologies. Our focus is on developing and refining assays and methods that offer more accurate, efficient, and accessible diagnostic solutions. Our commitment to enhancing public health and advancing medical research is reflected in our wide range of publications.
Here, we proudly present our key research findings and innovations.
COVID Detection
1. Bulterys PL, Garamani N, Stevens B, Sahoo MK, Huang C, Hogan CA, Zehnder J, Pinsky BA. Comparison of a laboratory-developed test targeting the envelope gene with three nucleic acid amplification tests for detection of SARS-CoV-2. J Clin Virol. 2020 Aug;129:104427. doi: 10.1016/j.jcv.2020.104427. Epub 2020 May 8. PMID: 32535398; PMCID: PMC7207111.
2. Kubina R, Dziedzic A. Molecular and Serological Tests for COVID-19 a Comparative Review of SARS-CoV-2 Coronavirus Laboratory and Point-of-Care Diagnostics. Diagnostics (Basel). 2020 Jun 26;10(6):434. doi: 10.3390/diagnostics10060434. PMID: 32604919; PMCID: PMC7345211.
3. Fellner MD, Bonaventura R, Basiletti J, Avaro M, Benedetti E, Campos A, Dattero ME, Russo M, Vladmirsky S, Molina V, Irazu L, Rodriguez MA, Pontoriero A, Cisterna DM, Baumeister EG. Evaluation of RT-qPCR and Loop-Mediated Isothermal Amplification (LAMP) Assays for the Detection of SARS-CoV-2 in Argentina. Genes (Basel). 2021 Apr 28;12(5):659. doi: 10.3390/genes12050659. PMID: 33924826; PMCID: PMC8146092.
4. Desai KT, Alfaro K, Mendoza L, Faron M, Mesich B, Maza M, Dominguez R, Valenzuela A, Acosta CD, Martínez M, Felix JC, Masch R, Gabrilovich S, Plump M, Novetsky AP, Einstein MH, Douglas NC, Cremer M, Wentzensen N. Multi-site clinical validation of Isothermal Amplification based SARS-COV-2 detection assays using different sampling strategies. medRxiv [Preprint]. 2021 Jul 6:2021.07.01.21259879. doi: 10.1101/2021.07.01.21259879. Update in: Microbiol Spectr. 2021 Oct 20;:e0084621. PMID: 34268516; PMCID: PMC8282105.
HPV Detection
1. Paytubi S, Taberna M, Mena M, Quirós B, Gomà M, Alemany L, Bosch FX, Pavón MÁ. The Isothermal Amplification AmpFire Assay for Human Papillomavirus (HPV) Detection and Genotyping in Formalin-Fixed, Paraffin-Embedded Oropharyngeal Cancer Samples. J Mol Diagn. 2022 Jan;24(1):79-87. doi: 10.1016/j.jmoldx.2021.10.008. Epub 2021 Nov 18. PMID: 34801703.
2. Connors KA, Abbott S, Jair K, Daniels J, Lintner M, Klein D, Wimpleberg A, Jordan JA. Cross comparison of AmpFire HPV genotyping assay and Roche human papillomavirus (HPV) linear array for HPV genotyping of anal swab samples. J Virol Methods. 2021 Jun;292:114113. doi: 10.1016/j.jviromet.2021.114113. Epub 2021 Feb 27. PMID: 33652016; PMCID: PMC8058289.
3. Murenzi G, Kim HY, Munyaneza A, Tuyisenge P, Zawadi TM, Buteera AM, Adedimeji A, Mutesa L, Castle PE, Anastos K, Palefsky JM. Anogenital Human Papillomavirus and HIV Infection in Rwandan Men Who Have Sex With Men. J Acquir Immune Defic Syndr. 2020 Aug 15;84(5):463-469. doi: 10.1097/QAI.0000000000002376. PMID: 32692104; PMCID: PMC7340233.
4. Murenzi G, Tuyisenge P, Kanyabwisha F, Munyaneza A, Muhoza B, Kubwimana G, Murangwa A, Mutesa L, Anastos K, Castle PE. Type-specific persistence, clearance and incidence of high-risk HPV among screen-positive Rwandan women living with HIV. Infect Agent Cancer. 2021 Feb 19;16(1):16. doi: 10.1186/s13027-021-00355-6. PMID: 33608036; PMCID: PMC7893720.
5. Zhang W, Du H, Huang X, Wang C, Duan X, Liu Y, Shi B, Zhang W, Qu X, Wei L, Schiffman M, Belinson JL, Wu R. Evaluation of an isothermal amplification HPV detection assay for primary cervical cancer screening. Infect Agent Cancer. 2020 Oct 23;15:65. doi: 10.1186/s13027-020-00328-1. PMID: 33110442; PMCID: PMC7583687.
6. Juliana NCA, Juma MH, Heijmans R, Ouburg S, Ali SM, Chauhan AS, Pemba AB, Sazawal S, Morré SA, Deb S, Ambrosino E. Detection of high-risk human papillomavirus (HPV) by the novel AmpFire isothermal HPV assay among pregnant women in Pemba Island, Tanzania. Pan Afr Med J. 2020 Oct 27;37:183. doi: 10.11604/pamj.2020.37.183.23367. PMID: 33447338; PMCID: PMC7778224.
7. Goldstein A, Lei Y, Goldstein L, Goldstein A, Bai QX, Felix J, Lipson R, Demarco M, Schiffman M, Egemen D, Desai KT, Bedell S, Gersten J, Goldstein G, O'Keefe K, O'Keefe C, O'Keefe T, Sebag C, Lobel L, Zhao A, Lu YL. A rapid, high-volume cervical screening project using self-sampling and isothermal PCR HPV testing. Infect Agent Cancer. 2020 Oct 22;15:64. doi: 10.1186/s13027-020-00329-0. PMID: 33106753; PMCID: PMC7579849.
8. Paytubi S, Taberna M, Mena M, Quirós B, Gomà M, Alemany L, Bosch FX, Pavón MÁ. The Isothermal Amplification AmpFire Assay for Human Papillomavirus (HPV) Detection and Genotyping in Formalin-Fixed, Paraffin-Embedded Oropharyngeal Cancer Samples. J Mol Diagn. 2022 Jan;24(1):79-87. doi: 10.1016/j.jmoldx.2021.10.008. Epub 2021 Nov 18. PMID: 34801703.
9. Acheampong LK, Effah K, Amuah JE, Tekpor E, Wormenor CM, Gedzah I, Kemawor S, Kachana AC, Danso PA, Essel NOM, Asomaning M, Agyiri D, Akakpo PK. Determining the prevalence of high-risk human papillomavirus infection using a novel cervical precancer screening approach in incarcerated women at the Nsawam Medium Security Prison, Ghana. Ecancermedicalscience. 2021 Jun 14;15:1248. doi: 10.3332/ecancer.2021.1248. PMID: 34267804; PMCID: PMC8241459.
10. Goldstein A, Lei Y, Goldstein L, Goldstein A, Bai QX, Felix J, Lipson R, Demarco M, Schiffman M, Egemen D, Desai KT, Bedell S, Gersten J, Goldstein G, O'Keefe K, O'Keefe C, O'Keefe T, Sebag C, Lobel L, Zhao A, Lu YL. A rapid, high-volume cervical screening project using self-sampling and isothermal PCR HPV testing. Infect Agent Cancer. 2020 Oct 22;15:64. doi: 10.1186/s13027-020-00329-0. PMID: 33106753; PMCID: PMC7579849.
11. Jang D, Shah A, Arias M, Ratnam S, Smieja M, Chen X, Wang Y, Speicher DJ, Chernesky M. Performance of AmpFire HPV assay on neck cervical lymph node aspirate and oropharyngeal samples. J Virol Methods. 2020 May;279:113840. doi: 10.1016/j.jviromet.2020.113840. Epub 2020 Feb 24. PMID: 32105752.
12. Effah K, Wormenor CM, Tekpor E, Amuah JE, Essel NOM, Gedzah I, Kemawor S, Hansen BT, Atuguba BH, Klutsey GB, Sesenu E, Danyo S, Akakpo PK. Evaluating operational parameters of the careHPV, GeneXpert, AmpFire, and MA-6000 HPV systems for cervical precancer screening: Experience from Battor, Ghana. PLOS Glob Public Health. 2023 Aug 25;3(8):e0001639. doi: 10.1371/journal.pgph.0001639. PMID: 37624774; PMCID: PMC10456161.
13. Belinson JL, Pretorius RG, Wu R. The AmpFire HPV technology. HPV World. 2024, No. 119
14. Bedell SL, Goldstein LS, Goldstein AR, Goldstein AT. Cervical Cancer Screening: Past, Present, and Future. Sex Med Rev. 2020 Jan;8(1):28-37. doi: 10.1016/j.sxmr.2019.09.005. Epub 2019 Nov 30. PMID: 31791846.
15. Sun J, Wu S, Hu L, Shang H, Yang Y, Pretorius R, Huang Y, Yang X, Wu X, Belinson J. Evaluation of Liquid Versus Dry Specimen Transport With a Newly Validated Isothermal Amplification High-Risk Human Papillomavirus Assay. J Low Genit Tract Dis. 2020 Jul;24(3):243-246. doi: 10.1097/LGT.0000000000000546. PMID: 32569253.
16. Arbyn M, Simon M, Peeters E, Xu L, Meijer CJLM, Berkhof J, Cuschieri K, Bonde J, Ostrbenk Vanlencak A, Zhao FH, Rezhake R, Gultekin M, Dillner J, de Sanjosé S, Canfell K, Hillemanns P, Almonte M, Wentzensen N, Poljak M. 2020 list of human papillomavirus assays suitable for primary cervical cancer screening. Clin Microbiol Infect. 2021 Aug;27(8):1083-1095. doi: 10.1016/j.cmi.2021.04.031. Epub 2021 May 8. PMID: 33975008.
17. Zhang W, Du H, Huang X, Wang C, Duan X, Liu Y, Shi B, Zhang W, Qu X, Wei L, Schiffman M, Belinson JL, Wu R. Evaluation of an isothermal amplification HPV detection assay for primary cervical cancer screening. Infect Agent Cancer. 2020 Oct 23;15:65. doi: 10.1186/s13027-020-00328-1. PMID: 33110442; PMCID: PMC7583687.
18. Vanderbilt CM, Bowman AS, Middha S, Petrova-Drus K, Tang YW, Chen X, Wang Y, Chang J, Rekhtman N, Busam KJ, Gupta S, Hameed M, Arcila ME, Ladanyi M, Berger MF, Dogan S, Zehir A. Defining Novel DNA Virus-Tumor Associations and Genomic Correlates Using Prospective Clinical Tumor/Normal Matched Sequencing Data. J Mol Diagn. 2022 May;24(5):515-528. doi: 10.1016/j.jmoldx.2022.01.011. Epub 2022 Mar 22. PMID: 35331965; PMCID: PMC9127461.
19. Murenzi G, Shumbusho F, Hansen N, Munyaneza A, Gage JC, Muhoza B, Kanyabwisha F, Pierz A, Tuyisenge P, Anastos K, Castle PE. Long-term human papillomavirus vaccination effectiveness and immunity in Rwandan women living with and without HIV: a study protocol. BMJ Open. 2022 Aug 25;12(8):e061650. doi: 10.1136/bmjopen-2022-061650. PMID: 36008069; PMCID: PMC9422845.
20. Kanyabwisha F, Kim HY, Shi Q, Murenzi G, Tuyisenge P, Kubwimana G, Munyaneza A, Murangwa A, Turizigiye O, Da Costa M, Nsengiyumva B, Chen X, Mutesa L, Anastos KM, Palefsky JM. Comparison of AmpFire and MY09/11 assays for HPV genotyping in anogenital specimen of Rwandan men who have sex with men. Heliyon. 2023 May 1;9(5):e16016. doi: 10.1016/j.heliyon.2023.e16016. PMID: 37305475; PMCID: PMC10256914.
21. Moyo S, Ramogola-Masire D, Moraka N, Tawe L, Noubary F, Motsumi K, Manowe G, Zuze B, Radibe B, Hungwe FT, Mohammed T, Maphorisa C, Shapiro R, Gaseitsiwe S, Luckett R. Comparison of the AmpFire Multiplex HPV Assay to the Xpert HPV Assay for detection of human papillomavirus and cervical disease in women with human immunodeficiency virus: A pragmatic performance evaluation. Res Sq [Preprint]. 2023 Mar 2:rs.3.rs-2606441. doi: 10.21203/rs.3.rs-2606441/v1. Update in: Infect Agent Cancer. 2023 May 10;18(1):29. PMID: 36909533; PMCID: PMC10002806.
22. Murangwa A, Desai KT, Gage JC, Murenzi G, Tuyisenge P, Kanyabwisha F, Musafili A, Kubwimana G, Mutesa L, Anastos K, Kim HY, Castle PE. Agreement between Xpert and AmpFire tests for high-risk human papillomavirus among HIV-positive women in Rwanda. Afr J Lab Med. 2022 Oct 19;11(1):1827. doi: 10.4102/ajlm.v11i1.1827. PMID: 36353194; PMCID: PMC9639372.
23. F Palma, M Bunker-Alberts, M Miskell, AB Penados, R Godfrey. Experience of Ampfire self-sampling technology in screening for cervical cancer in Guatemala. HPV World. 2024. No. 229
24. Effah K, Wormenor CM, Tekpor E, Amuah JE, Essel NOM, Gedzah I, Kemawor S, Hansen BT, Atuguba BH, Klutsey GB, Sesenu E, Danyo S, Akakpo PK. Evaluating operational parameters of the careHPV, GeneXpert, AmpFire, and MA-6000 HPV systems for cervical precancer screening: Experience from Battor, Ghana. PLOS Glob Public Health. 2023 Aug 25;3(8):e0001639. doi: 10.1371/journal.pgph.0001639. PMID: 37624774; PMCID: PMC10456161.
25. Effah K, Tekpor E, Wormenor CM, Amuah JE, Essel NO, Atuguba BH, Klutsey GB, Sesenu E, Tay G, Tibu F, Kemawor S, Gedzah I, Morkli EAC, Danyo S, Akakpo PK. Concurrent HPV DNA testing and a visual inspection method for cervical precancer screening: A practical approach from Battor, Ghana. PLOS Glob Public Health. 2023 Apr 20;3(4):e0001830. doi: 10.1371/journal.pgph.0001830. PMID: 37079498; PMCID: PMC10118129.
26. Chachage M, Parikh AP, Mahenge A, Bahemana E, Mnkai J, Mbuya W, Mcharo R, Maganga L, Mwamwaja J, Gervas R, Kibuuka H, Maswai J, Singoei V, Iroezindu M, Fasina A, Esber A, Dear N, Imbach M, Crowell TA, Hern J, Song X, Hoelscher M, Polyak CS, Ake JA, Geldmacher C; AFRICOS Study Group. High-risk human papillomavirus genotype distribution among women living with and at risk for HIV in Africa. AIDS. 2023 Mar 15;37(4):625-635. doi: 10.1097/QAD.0000000000003437. Epub 2022 Dec 1. PMID: 36398743; PMCID: PMC9994804.
27. Desai KT, Adepiti CA, Schiffman M, Egemen D, Gage JC, Wentzensen N, de Sanjose S, Burk RD, Ajenifuja KO. Redesign of a rapid, low-cost HPV typing assay to support risk-based cervical screening and management. Int J Cancer. 2022 Oct 1;151(7):1142-1149. doi: 10.1002/ijc.34151. Epub 2022 Jun 24. PMID: 35666530; PMCID: PMC9378567.
28. Inturrisi F, de Sanjosé S, Desai KT, Dagnall C, Egemen D, Befano B, Rodriguez AC, Jeronimo JA, Zuna RE, Hoffman A, Farhat Nozzari S, Walker JL, Perkins RB, Wentzensen N, Palefsky JM, Schiffman M. A rapid HPV typing assay to support global cervical cancer screening and risk-based management: A cross-sectional study. Int J Cancer. 2024 Jan 15;154(2):241-250. doi: 10.1002/ijc.34698. Epub 2023 Sep 29. PMID: 37772799.
29. de Sanjosé S, Perkins RB, Campos NG, Inturrisi F, Egemen D, Befano B, Rodriguez AC, Jerónimo J, Cheung LC, Desai K, Han P, Novetsky AP, Ukwuani A, Marcus J, Ahmed SR, Wentzensen N, Kalpathy-Cramer J, Schiffman M; PAVE Study Group. Design of the HPV-Automated Visual Evaluation (PAVE) Study: Validating a Novel Cervical Screening Strategy. medRxiv [Preprint]. 2023 Oct 23:2023.08.30.23294826. doi: 10.1101/2023.08.30.23294826. PMID: 37693492; PMCID: PMC10491363.
30. Zhang Y, Zhang W, Du H, Qu X, Chen Y, Wang J, Wu R. A comparative analysis of cycle threshold (Ct) values from Cobas4800 and AmpFire HPV assay for triage of women with positive hrHPV results. BMC Infect Dis. 2023 Nov 10;23(1):783. doi: 10.1186/s12879-023-08737-4. PMID: 37950154; PMCID: PMC10636838.
31. Chan AHY, Ngu SF, Lau LSK, Tsun OKL, Ngan HYS, Cheung ANY, Chan KKL. Evaluation of an Isothermal Amplification HPV Assay on Self-Collected Vaginal Samples as Compared to Clinician-Collected Cervical Samples. Diagnostics (Basel). 2023 Oct 24;13(21):3297. doi: 10.3390/diagnostics13213297. PMID: 37958193; PMCID: PMC10647996.
32. Shin MB, Liu G, Mugo N, Garcia PJ, Rao DW, Bayer CJ, Eckert LO, Pinder LF, Wasserheit JN, Barnabas RV. A Framework for Cervical Cancer Elimination in Low-and-Middle-Income Countries: A Scoping Review and Roadmap for Interventions and Research Priorities. Front Public Health. 2021 Jul 1;9:670032. doi: 10.3389/fpubh.2021.670032. PMID: 34277540; PMCID: PMC8281011.
33. Glinska P, Komerska K, Janik B, Olkowicz J, Jedrzejewska I, Macios A, Wieszczy P, Kaminski MF, Arbyn M, Nowakowski A. HPV testing in Polish population-based cervical cancer screening programme (HIPPO project)-study protocol of a randomised healthcare policy trial. BMC Cancer. 2023 Nov 17;23(1):1118. doi: 10.1186/s12885-023-11597-5. PMID: 37978452; PMCID: PMC10655392.
34. Fang SH, Plesa M, Carchman EH, Cowell NA, Staudt E, Twaroski KA, Buchwald UK, Trimble CL. A phase I study of intra-anal artesunate (suppositories) to treat anal high-grade squamous intraepithelial lesions. PLoS One. 2023 Dec 15;18(12):e0295647. doi: 10.1371/journal.pone.0295647. PMID: 38100463; PMCID: PMC10723659.
Non-invasive Prenatal Testing
1. Haidong W, Zhijie Y, Picchiassi E, et al. Non-invasive prenatal testing of fetal aneuploidies using a new method based on digital droplet PCR and cell free fetal DNA. medRxiv; 2020. DOI: 10.1101/2020.12.19.20248553.
2. Practice Bulletin No. 162: Prenatal Diagnostic Testing for Genetic Disorders. Obstet Gynecol. 2016 May;127(5):e108-e122. doi: 10.1097/AOG.0000000000001405. PMID: 26938573.
3. Dopico PJ, Le MN, Burgess B, Yang Z, Zhao Y, Wang Y, George TJ, Fan ZH. Longitudinal Study of Circulating Biomarkers in Patients with Resectable Pancreatic Ductal Adenocarcinoma. Biosensors (Basel). 2022 Mar 30;12(4):206. doi: 10.3390/bios12040206. PMID: 35448266; PMCID: PMC9028387.