Atila Multiplex Digital PCR Quantification Assay

Absolute quantification of clinically important diseases using digital PCR technology.

Oncology
Reproductive Health including NIPT
Infectious Disease Testing (IDT)
Absolute Quantification
Extraction Free
Simplest dPCR Test for Infectious Diseases
Fastest dPCR Test, Results Within an Hour

Each kit includes 100 tests.

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Category: Multiplex Digital PCR Platform

Atila Multiplex Digital PCR Quantification Assay

Digital PCR (dPCR) is a cutting-edge molecular technology designed for the absolute quantification of nucleic acids with unmatched precision and sensitivity. After partitioning the sample, each reaction undergoes end-point PCR. Partitions are then analyzed for fluorescence to determine whether a target is present (positive) or absent (negative). This binary readout enables absolute quantification of target molecules without the need for standard curves. As a result, digital PCR (dPCR) offers greater precision and sensitivity compared to traditional qPCR. This enables more confident clinical decisions with regards to accurate diagnosis of various infections or abnormalities.

Atila has created a digital platform for analysis of clinically important diseases. Our ready-to-use, simple and extraction-free workflow reduces lab hands on time making this product an ideal choice to scale up DNA quantification projects and it allows for quantification and screening for multiple targets in a single well. The results are delivered in 1 hour and multiple patient samples can be analyzed in a single plate for high- throughput testing. Our assay provides superior sensitivity and specificity paving the way for accurate diagnosis of various diseases and conditions.

Features Include

Superior Performance

Allows quantification and screening for multiple mutations in a single well.

Fast Turnaround Time

Delivers results within an hour, ideal for high-throughput testing.

High Sensitivity

Provides sensitive and precise detection down to 0.5 cp/uL in a single well.

Ready-to-Use Reagents

Minimize preparation time.

Extraction-Free Workflow

No need for RNA/DNA extraction, streamlining the process.

Technical Specs

Assay Targets

Multiple targets in each assay. Check the available products section for full list of targets.

Reaction Time

Assays require <20 min hands on time and 1 hour of instrument time.

Sample Types

Assay dependent.

Instruments

Seamlessly compatible with Qiagen QIAcuity platform.

Workflow Overview for the Atila Multiplex Digital PCR Quantification Assay

This section is for demonstrative purposes only and may be incomplete or inaccurate. Always refer to the product instructions for precise guidelines and directions.

Step 1

Data Analysis

Performance of Atila Multiplex Digital PCR Quantification Assay

Data Shows:

1) capability of absolute quantification

2) good linearity

3) capability of detecting multi-positive samples

(1) Detection of HPV in Positive Clinical Samples with quantification

(2) 1D Scatter Plot For HPV45 Positive Samples Demonstrating Ideal Linearity

(3) 2D Scatter Plot of A Multi Positive Sample Analyzed on dPCR(this sample is co-infected with HPV16 and HPV18)

Available Infectious Disease Testing Products (click to see targets)

iAMP Digital Respiratory Panel (diAMP-RPP-100)

Detects:
Respiratory syncytial virus, Influenza A, Influenza B, SARS-CoV-2, Rhinovirus, Enterovirus, Coronavirus (229E, HKU1, OC43, NL63), Parainfluenza virus (1-4), Human adenovirus, Human bocavirus, Human metapneumovirus, Mycoplasma pneumonia, Klebsiella pneumonia, Bordetella parapertussis, Bordetella pertussis, Streptococcus pneumonia, Haemophilus influenzae, Legionella pneumophila, Chlamydia pneumonia

iAMP Digital Covid-19 Detection Kit (diAMP-COVID19-100)

Detects:
SARS-CoV-2 (N and ORF gene without differentiation).

iAMP Digital Covid-19 SANO Assay (diAMP-COVSANO-100)

Detects:
SARS-CoV-2 (N and ORF gene without differentiation)

iAMP Digital COV2/INF Detection Kit (diAMP-COVFLU-100)

Detects:
SARS-CoV-2, Influenza, A Influenza B

iAMP Digital COV/FLU/RSV Detection Kit (diAMP-COVFLURSV-100)

Detects:
SARS-CoV-2, Influenza, A Influenza B, RSV A/B

iAMP Digital INF/RSV Detection Kit (diAMP-INFRSV-100)

Detects:
Influenza A, Influenza B, Respiratory syncytial virus (RSV)

iAMP Digital UISolution Assay (diAMP-UTI-100)

Detects:
Acinetobacter baumannii, Citrobacter spp., Enterobacter cloacae, Enterococcus spp.,, Escherichia coli, Klebsiella aerogenes, Klebsiella oxytoca, Klebsiella pneumoniae, Morganella morganii, Proteus spp., Providencia stuartii, Pseudomonas aeruginosa, Staphylococcus saprophyticus, Streptococcus agalactiae, Candida albicans

iAMP Digital Wound Panel (diAMP-WD-100)

Detects:
Bacteria- Acinetobacter baumannii, Anaerococcus spp,, Bacteroides fragilis, Citrobacter spp., Enterococcus spp., Escherichia coli, Klebsiella oxytoca, Klebsiella pneumoniae, Proteus spp., Pseudomonas aeruginosa, Serratia marcescens, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus agalactiae, Streptococcus pyogenes
Virus- Herpes simplex virus
Fungi- Candida spp.. Candida glabrata, Candida krusei

Atila Digital Nail Fungus Panel (dAt-NF-100)

Detects:
Trichophyton spp., Aspergillus, Curvularia spp., Fusarium solani, Epidermophyton floccosum, Cryptococcus, Malassezia spp., Sarocladium strictum, Scopulariopsis brevicaulis. Microsporum audouinii/canis Alternaria, NeoScytalidim d/h, Geotrichum candidum, Candida spp., Trichosporon Neofusicoccum mangiferae

iAMP Digital Vaginal Panel (diAMP-VAG-100)

Detects:
Bacteria- Bacterial Vaginosis Associated Bacteria-2, Megasphaera-1 Megasphaera-2, Trichomonas vaginalis, Atopobium vaginae
Fungi- Candida albicans, Candida dubliniensis, Candida tropicalis, Candida parapsilosis, Candida krusei, Candida glabrata

iAMP Digital Drug Resistance Panel (diAMP-DRFP-100)

Detects:
KPC, NDM, OXA-48, IMP, VIM, CTX, VanA, VanB, MecA, SUL1, SUL2, SUL3, Qnrs, dfrA1, dfrA5, dfrA12, dfrA17 MefA, MrsA, ermA, ermB, ermC, mphA, and ereA

iAMP Digital C. auris Detection Kit (diAMP-CAU-100)

Detects:
Candida auris

ScreenFire Digital HPV RS Kit (dM5FHPV-100-S )

Detects:
HPV16, HPV18/45, HPV31/33/35/52/58 and HPV39/51/56/59/68

AmpFire Digital HPV Screening 16/18/HR (dMHPVF1618-100)

Detects:
HPV genotypes 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 and simultaneously identify HPV16 and HPV18.

AmpFire Digital HPV High Risk Genotyping (dGHPVF-100)

Detects:
HPV 16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68

AmpFire Digital Monkeypox Assay (dMPX-100-RUO)

Detects:
Detects Monkeypox virus (MPXV) in subtypes from the West African clade and the Congo Basin (Central African) clade

AmpFire Digital Malaria Assay (dMAL-100-RUO)

Detects:
Plasmodium falciparum, Plasmodium knowlesi ,Plasmodium malariae, Plasmodium ovale, and Plasmodium vivax

AmpFire Digital Tuberculosis (TB) Assay (dMTB-100-RUO)

Detects:
Mycobacterium tuberculosis, Mycobacterium bovis, Mycobacterium africanum, Mycobacterium canettii and Mycobacterium microti

iAMP Digital H5N1 Detection Kit (diAMP-H5N1-100)

Detects:
H5N1

iAMP Digital Syphilis Detection Kit (diAMP-TP-100)

Detects:
Treponema pallidum subspecies pallidum

iAMP Digital Sepsis Detection Kit (diAMP-SEP-100)

Detects:
Panel Pathogens- Escherichia coli,,Enterococcus faecalis,, Candida spp.2, Serratia marcescens, Enterococcus faecium, Candida krusei, Staphylococcus epidermidis, Enterococcus gallinarum, Candida glabrata, Staphylococcus aureus, Stenotrophomonas maltophilia, Streptococcus pneumoniae, Klebsiella pneumoniae, Streptococcus agalactea, Enterobacter cloacae, Klebsiella oxytoca, Acinetobacter baumannii, Haemophilus influenzae, Klebsiella aerogenes, Pseudomonas aeruginosa, Neisseria meningitidis, Bacteroides fragilis, Streptococcus pyogenes, Proteus mirabilis, Candida auris, Staphylococcus lugdunensis, Cryptococcus neoformans gattii, Listeria monocytogenes, Salmonella spp.1 Streptococcus mitis
Drug Resistance Markers- CTX, OXA-48, IMP, NDM, VIM, MecA, KPC, VanA, VanB, TEM, MCR-1
1 Primer design is based on the entire genus
2 Candida albicans, Candida tropicalis, Candida parapsilosis, and Candida dubliniensis.

iAMP Digital Group A Streptococcus Detection Kit (diAMP-GAS-100)

Detects:
Streptococcus pyogenes

iAMP Digital Bacterial Meningitis Panel (diAMP-BMS-100)

Detects:
Pathogens: H. influenzae, L. monocytogenes, N. meningitidis, S. agalactiae, S. pneumoniae, E. coli, C. neoformans
Drug resistance gene markers: carbapenemase genes (NDM, KPC, OXA-48, VIM, IMP), extended spectrum beta-lactamase (ESBL) gene (CTX-M1), vancomycin resistance genes (VanA, VanB), oxacillin/methicillin resistance gene (MecA), sulfanamide resistant genes (SUL1, SUL2, SUL3), trimethoprim resistant genes (dfrA1, dfrA5, dfrA12, dfrA17), plasmid-mediated quinolone resistance marker (QnrS), and Marcolide resistant genes (MefA, MrsA, ermA, ermB, ermC, ereA, mphA).

iAMP Digital Streptococcus Pneumoniae Detection Kit (diAMP-SPne-100)

Detects:
Streptococcus pneumoniae (S. pneumoniae)

iAMP Digital Group B Streptococcus Detection Kit (diAMP-GBS-100)

Detects:
Streptococcus agalactiae (S. agalactiae)

iAMP Digital HSV 1/2 Detection Kit (diAMP-HSV-100)

Detects:
Herpes simplex virus type 1 and 2

iAMP Digital Candidiasis Detection Kit (diAMP-CAN-100)

Detects:
Candida albicans, Candida dubliniensis, Candida tropicalis, Candida parapsilosis, Candida glabrata, and Candida krusei

iAMP Digital Bacterial Vaginosis Detection Kit (diAMP-BV-100)

Detects:
Gardnerella vaginalis (GV), Atopobium vaginae (AV), Bacterial Vaginosis Associated Bacteria-2 (BVAB-2), Megasphaera-1 and Megasphaera-2 (Mega1 and Mega2)

iAMP Digital Ureaplasma Detection Kit (diAMP-UUUP-100)

Detects:
Ureaplasma urealyticum (UU), Ureaplasma parvum (UP)

iAMP Digital Mycoplasma Hominis Detection Kit (diAMP-MH-100)

Detects:
Mycoplasma hominis

iAMP Digital CT/NG/TV/MG Detection Kit (diAMP-4STI-100)

Detects:
Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis, Mycoplasma genitalium

Atila Digital Women's Health Panel (dATWH)

Detects:
ATWH CT/NG/TV/MG Detection Kit: Chlamydia trachomatis, Trichomonas vaginalis, Mycoplasma genitalium, Neisseria gonorrhoeae
ATWH HSV 1/2 Detection Kit: Herpes Simplex Virus type 1, Herpes Simplex Virus type 2
ATWH Candidiasis Detection Kit: Candida albicans, Candida dubliniensis, Candida tropicalis, Candida parapsilosis, Candida glabrata, Candida krusei
ATWH MH Detection Kit: Mycoplasma Hominis
ATWH Ureaplasma Detection Kit: Ureaplasma urealyticum, Ureaplasma parvum
ATWH Bacterial Vaginosis Detection Kit: Gardnerella vaginalis, Atopobium vaginae, Bacterial Vaginosis Associated Bacteria-2, Megasphaera-1, Megasphaera-2
ATWH Internal Control Detection Kit: Homo Sapiens

iAMP Digital NG Resistance Assay (diAMP-NGResis-100)

Detects:
gyrA gene with S91F mutation from Neisseria gonorrhea

iAMP Digital MG Resistance Assay (diAMP-MGResis-100)

Detects:
mutations at positions 2058 and 2059 in the 23S rRNA gene (A2058G, A2059G, A2058T, A2058C, and A2059C, Escherichia coli numbering) from Mycoplasma genitalium (MG)

iAMP Digital CT Detection Kit (diAMP-CT-100)

Detects:
Chlamydia trachomatis

Available Oncology Testing Products (click to see targets)

Lung Cancer Mutation Screening Kit (ACPD-LN-100)

Identifies 108 possible mutation sites in four groups

Colorectal Cancer Mutation Screening (ACPD-CR-100)

Identifies 122 possible mutation sites in four groups

EGFR Multiplex Mutation Screening KIt (ACPD-EG-100)

Identifies 64 possible mutation sites including G719X, Ex19Del, S768I, Ex20Ins, T790M, L858R and L861Q mutation groups

BRAF Multiplex Mutation Screening Kit (ACPD-BR-100)

Identifies 62 possible mutation sites

KRAS Multiplex Mutation Screening Kit (ACPD-KR-100)

Identifies 61 possible mutation sites including KRAS exon 2, 3 and 4 mutation groups.

NRAS Multiplex Mutation Screening Kit (ACPD-NR-100)

Identifies 35 possible mutation sites.

PIK3CA Multiplex Mutation Screening Kit (ACPD-PI-100)

Identifies as many as 40 possible mutation sites.

RET/MET Multiplex Mutation Screening Kit (ACPD-RE-100)

Identifies as many as 11 possible mutation sites.

HER2 Multiplex Mutation Screening Kit (ACPD-HE-100)

Identifies 18 possible mutation sites including p.G776 > VC, p.P780_Y781insGSP, p.V777_G778insCG, p.L755S and p.V777L.

TP53 Multiplex Mutation Screening Kit (ACPD-TP53-100)

Identifies 295 possible mutation sites.

APC Multiplex Mutation Screening Kit (ACPD-AP-100)

Identifies 38 possible mutation sites.

Available Reproductive Health Testing (click to see targets)

iSAFE dPCR Rhesus D Genotyping Kit (dRhDGT-100Q-RUO)

Detects:
Exon 4, exon 4 pseudogene form, exon 5 and exon 7 on chromosome 1.

iSAFE Non-invasive Prenatal Test Kit (NIPT-100Q)

Detects:
Trisomy 21, Trisomy 18, Trisomy 13, Fetal Gender, Fetal Fraction.

iSAFE Non-Invasive Prenatal Test Comprehensive Kit (NIPT-100QS)

Detects:
Trisomy 21, 18, 13, sex chromosome aneuploidy, fetal fraction, and gender determination.

ISAFE Fetal Fraction Determination Assay (NIPT-FF-100Q)

Detects:
Fetal Fraction

iSAFE™ NIPT Gender and Fetal Fraction Determination Assay Kit (NIPT-Y-100Q)

Simultaneously quantifies cfDNA, determines fetal gender, and measures fetal fraction for male fetus in the maternal blood cfDNA.

iSAFE™ NIPT Genomic DNA Contamination Determination Assay Kit (NIPT-GS-100Q)

Detects:
Genomic DNA contamination

iSAFE PGT-A Kit (PGTA-100)

Quantifies the copy numbers of all 24 chromosomes, plus the 22q deletion region after whole genome amplification (WGA).

iSAFE Microdeletions Testing Kit (MDT5-100)

Quantifies the microdeletions on the chromosomes 4, 5, 7, 15, and 22

Atila Multiplex Digital PCR Quantification Assay

Atila Multiplex Digital PCR Quantification Assay