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Streptococcus Agalactiae Real-Time PCR Detection Kit

This kit is designed for rapid qualitative detection of S. agalactiae DNA isolated from patient samples.

Each kit includes 100 reactions.

This product is for research use only.

Category: SKU: AP-GBS-100

Streptococcus Agalactiae Real-Time PCR Detection Kit

The Streptococcus Agalactiae Real-Time PCR Detection Kit provides a PCR master mix which contains reagents and enzymes for PCR amplification and a primer mix which are specific to the S. agalactiae genome. In addition, the kit provides an internal control to identify possible PCR inhibition or sample preparation failure.

Streptococcus agalactiae, commonly known as Group B Streptococcus (GBS), is a gram-positive coccus with both commensal and pathogenic attributes. While often residing asymptomatically in the human gastrointestinal and genitourinary tracts, GBS can cause concern in certain vulnerable populations.

In newborns, it can lead to life-threatening infections such as sepsis, pneumonia, and meningitis, transmitted from colonized mothers during childbirth. S. agalactiae can also cause infections among adults, including urinary tract infections, skin and soft tissue infections, and bone and joint infections.

Its ability to swiftly adapt to different host environments and potential to induce a range of clinical manifestations make it a subject of both clinical and research interest.

Features Include

Rapid Diagnosis

Results in 2 hours. All reagents are included in the kit. Minimal hands-on time.

Easy to Use

Real-time and melt curve analysis. Easy to read and interpret results.

Excellent Performance

Novel PCR technology ensures high sensitivity and high specificity.

Cost Effective

SYBR green based PCR reaction greatly reduces cost.

Technical Specs

Assay Targets

Streptococcus agalactiae (S. agalactiae)

Reaction Time

About 2 hours.

Sample Types

purified DNA

Sensitivity

10 copies per reaction.

Instruments

Compatible with Bio-Rad CFX96, iCycler, ABI7500, Stratagene Mx3000P/Mx3005P, and other commonly used RT-qPCR instruments.

Workflow Overview for the Streptococcus Agalactiae Real-Time PCR Detection Kit

This section is for demonstrative purposes only and may be incomplete or inaccurate. Always refer to the product instructions for precise guidelines and directions.

Prepare the PCR reaction mix by combining 12.5μL x (N+2) of PCR master mix with 5μL x (N+2) of primer mix.
Finalize by adding H2O 5.5μL x (N+2) to complete the reaction mix.
Add 23μL of reaction mix per well along with 2μL of extracted DNA and internal control.
Perform PCR.
Result in about 2 hours.
SIMPLE. FAST. AFFORDABLE.

Please contact us for a quote or any questions about our Streptococcus Agalactiae Real-Time PCR Detection Kit.

Streptococcus Agalactiae Real-Time PCR Detection Kit
Sample Types

  • Urine specimens processed and tested with iAMP UISoluton Assay.
  • Direct urine specimens without any preservatives.Note: Clean-catch urine specimens are highly recommended.
  • Direct dry wound swab specimens collected by clinicians with Atila Swab Sample Collection Device (iAMP-SWA-SCD). Alternatively, specimens collected with sample collection devices from other vendors may be used after self-validation.
  • Wound swab specimens collected in transport medium (with sample collection devices from other vendors) may be used after self-validation.

Streptococcus Agalactiae Real-Time PCR Detection Kit
Sample Types

  • Vaginal/cervical swab collected as dry swabs or in 1X iAMP Sample Buffer.
  • Vaginal/cervical swab collected in commonly used transport medium.
  • First-void urine specimen without any preservatives.

Streptococcus Agalactiae Real-Time PCR Detection Kit
Sample Types

  • Vaginal/cervical swab collected as dry swabs or in 1X iAMP Sample Buffer, or in commonly used transport medium.
  • First-void urine specimen without any preservatives.
  • Ocular swab collected as dry swabs or in 1X iAMP Sample Buffer, or in commonly used transport medium.

Streptococcus Agalactiae Real-Time PCR Detection Kit
Assay Targets

  • Trichophyton spp.
  • Aspergillus
  • Curvularia spp.
  • Fusarium solani
  • Epidermophyton floccosum
  • Cryptococcus
  • Malassezia spp.
  • Sarocladium strictum
  • Scopulariopsis brevicaulis
  • Microsporum audouinii/canis
  • Alternaria
  • NeoScytalidim d/h
  • Geotrichum candidum
  • Candida spp.
  • Trichosporon
  • Neofusicoccum mangiferae

iAMP UISolution Assay for UTIs
Assay Targets

  • Acinetobacter baumannii
  • Citrobacter spp.
  • Enterobacter cloacae
  • Enterococcus spp.
  • Escherichia coli
  • Klebsiella aerogenes
  • Klebsiella oxytoca
  • Klebsiella pneumoniae
  • Morganella morganii
  • Proteus spp.
  • Providencia stuartii
  • Pseudomonas aeruginosa
  • Staphylococcus saprophyticus
  • Streptococcus agalactiae
  • Candida albicans
Pathogen TypeSpecies
BacteriaAcinetobacter baumannii
Anaerococcus spp
Bacteroides fragilis
Citrobacter spp.
Enterococcus spp.
Escherichia coli
Klebsiella oxytoca
Klebsiella pneumoniae
Proteus spp.
Pseudomonas aeruginosa
Serratia marcescens
Staphylococcus aureus
Staphylococcus epidermidis
Streptococcus agalactiae
Streptococcus pyogenes
VirusHerpes simplex virus 1
FungiCandida spp.
Candida glabrata
Candida krusei

Streptococcus Agalactiae Real-Time PCR Detection Kit
Assay Targets

  • Respiratory syncytial virus
  • Influenza A
  • Influenza B
  • SARS-CoV-2
  • Rhinovirus
  • Enterovirus
  • Coronavirus (229E, HKU1, OC43, NL63)
  • Parainfluenza virus (1-4)
  • Human adenovirus
  • Human bocavirus
  • Human metapneumovirus
  • Mycoplasma pneumonia
  • Klebsiella pneumonia
  • Bordetella parapertussis
  • Bordetella pertussis
  • Streptococcus pneumonia
  • Haemophilus influenzae
  • Legionella pneumophila
  • Chlamydia pneumonia
Detection Kit Species
ATWH CT/NG/TV/MG Detection Kit Chlamydia trachomatis
Trichomonas vaginalis
Mycoplasma genitalium
Neisseria gonorrhoeae
ATWH HSV 1/2 Detection Kit Herpes Simplex Virus type 1
Herpes Simplex Virus type 2
ATWH Candidiasis Detection Kit Candida albicans
Candida dubliniensis
Candida tropicalis
Candida parapsilosis
Candida glabrata
Candida krusei
ATWH MH Detection Kit Mycoplasma Hominis
ATWH Ureaplasma Detection Kit Ureaplasma urealyticum
Ureaplasma parvum
ATWH Bacterial Vaginosis Detection Kit Gardnerella vaginalis
Atopobium vaginae
Bacterial Vaginosis Associated Bacteria-2
Megasphaera-1
Megasphaera-2
ATWH Internal Control Detection Kit Homo Sapiens