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Nucleic Acid Extraction Kit (Magnetic Bead Method)

The Atila Nucleic Acid Extraction Kit is intended for the extraction, enrichment, and purification of nucleic acid (DNA/RNA) from samples such as serum, plasma, nasopharyngeal swab, cell preservation fluid, tissue fluid, urine, and secretions.

Four kits are available.

  • A200-32: Manual extraction kit, enhanced performance for cfDNA extraction.
  • B200-32: Automated extraction kit, universal, max input 200ul per sample, elute in 50ul.
  • C200-32: Automated extraction kit, enhanced performance for cfDNA extraction, max input 200ul per sample, elute in 50ul.
  • D200-32: Manual extraction kit, universal.

Each kit includes 32 tests.

This product is for research use only in the US.

Category: SKU: B200-32

Nucleic Acid Extraction Kit (Magnetic Bead Method)

The Atila Nucleic Acid Extraction Kit (Magnetic Bead Method) ensures reproducible extraction of DNA/RNA. The procedure comprises 3 simple steps: lyse, wash, and elute. The DNA/RNA binds to the surface of magnetic beads while sample lysis. During the wash steps, contaminants and PCR inhibitors are effectively removed, and pure DNA/RNA is eluted in the elution buffer.

Features Include

Kit Flexibility

One kit works with a variety of sample types and sizes.

Ultraclean DNA/RNA

Minimal contamination of extracted sample means successful downstream application.

Improved Design

Optimized design and buffer formulation for better yield and purity.

High Throughput Compatible

Easy to automate on the Atila-Pure32 instrument for high-throughput sample handling.

Technical Specs

Assay Targets

DNA/RNA

Reaction Time

Kits A200-32 & D200-32 are manual. B200-32 & C200-32 are less than 25 minutes of instrument time.

Sample Types

Serum, plasma, nasopharyngeal swab, cell preservation fluid, tissue fluid, urine and secretions.

Sensitivity

Can extract almost 100% of DNA/RNA in the sample.

Instruments

Atila-Pure32 and other programmable extraction instruments. For manual extraction kit, a dry bath heating block and magnetic rack are required but not provide with the kit.

Auto Workflow

Workflow Overview for the Nucleic Acid Extraction Kit (Magnetic Bead Method)

This section is for demonstrative purposes only and may be incomplete or inaccurate. Always refer to the product instructions for precise guidelines and directions.

Take out all the components from the kit, turn the 96-well pates upside down and mix the liquid well.
Add Proteinase K and Sample to the position A1-H1 and A7-H7 in order.
Put the 96-well plates into the corresponding position of the instrument and start the program.
Take out 96-well plates and pipette extracted samples from the position A6-H6 and A12-H12 for following operation.

Manual Workflow

Workflow Overview for the Nucleic Acid Extraction Kit (Magnetic Bead Method)

This section is for demonstrative purposes only and may be incomplete or inaccurate. Always refer to the product instructions for precise guidelines and directions.

Mix Sample with Lysis Buffer, magnetic Beads Solution and Proteinase K solution.
Heat the sample for 10 min on a dry bath.
Separate the beads on a magnetic rack and wash with washing buffer.
Elute in Elution Buffer on a dry bath.
Separate the beads on a magnetic rack and store the eluted sample for future usage.
SIMPLE. FAST. AFFORDABLE.

Please contact us for a quote or any questions about our iAMP Automatic Instrument.

Nucleic Acid Extraction Kit (Magnetic Bead Method)
Sample Types

  • Urine specimens processed and tested with iAMP UISoluton Assay.
  • Direct urine specimens without any preservatives.Note: Clean-catch urine specimens are highly recommended.
  • Direct dry wound swab specimens collected by clinicians with Atila Swab Sample Collection Device (iAMP-SWA-SCD). Alternatively, specimens collected with sample collection devices from other vendors may be used after self-validation.
  • Wound swab specimens collected in transport medium (with sample collection devices from other vendors) may be used after self-validation.

Nucleic Acid Extraction Kit (Magnetic Bead Method)
Sample Types

  • Vaginal/cervical swab collected as dry swabs or in 1X iAMP Sample Buffer.
  • Vaginal/cervical swab collected in commonly used transport medium.
  • First-void urine specimen without any preservatives.

Nucleic Acid Extraction Kit (Magnetic Bead Method)
Sample Types

  • Vaginal/cervical swab collected as dry swabs or in 1X iAMP Sample Buffer, or in commonly used transport medium.
  • First-void urine specimen without any preservatives.
  • Ocular swab collected as dry swabs or in 1X iAMP Sample Buffer, or in commonly used transport medium.

Nucleic Acid Extraction Kit (Magnetic Bead Method)
Assay Targets

  • Trichophyton spp.
  • Aspergillus
  • Curvularia spp.
  • Fusarium solani
  • Epidermophyton floccosum
  • Cryptococcus
  • Malassezia spp.
  • Sarocladium strictum
  • Scopulariopsis brevicaulis
  • Microsporum audouinii/canis
  • Alternaria
  • NeoScytalidim d/h
  • Geotrichum candidum
  • Candida spp.
  • Trichosporon
  • Neofusicoccum mangiferae

iAMP UISolution Assay for UTIs
Assay Targets

  • Acinetobacter baumannii
  • Citrobacter spp.
  • Enterobacter cloacae
  • Enterococcus spp.
  • Escherichia coli
  • Klebsiella aerogenes
  • Klebsiella oxytoca
  • Klebsiella pneumoniae
  • Morganella morganii
  • Proteus spp.
  • Providencia stuartii
  • Pseudomonas aeruginosa
  • Staphylococcus saprophyticus
  • Streptococcus agalactiae
  • Candida albicans
Pathogen TypeSpecies
BacteriaAcinetobacter baumannii
Anaerococcus spp
Bacteroides fragilis
Citrobacter spp.
Enterococcus spp.
Escherichia coli
Klebsiella oxytoca
Klebsiella pneumoniae
Proteus spp.
Pseudomonas aeruginosa
Serratia marcescens
Staphylococcus aureus
Staphylococcus epidermidis
Streptococcus agalactiae
Streptococcus pyogenes
VirusHerpes simplex virus 1
FungiCandida spp.
Candida glabrata
Candida krusei

Nucleic Acid Extraction Kit (Magnetic Bead Method)
Assay Targets

  • Respiratory syncytial virus
  • Influenza A
  • Influenza B
  • SARS-CoV-2
  • Rhinovirus
  • Enterovirus
  • Coronavirus (229E, HKU1, OC43, NL63)
  • Parainfluenza virus (1-4)
  • Human adenovirus
  • Human bocavirus
  • Human metapneumovirus
  • Mycoplasma pneumonia
  • Klebsiella pneumonia
  • Bordetella parapertussis
  • Bordetella pertussis
  • Streptococcus pneumonia
  • Haemophilus influenzae
  • Legionella pneumophila
  • Chlamydia pneumonia
Detection Kit Species
ATWH CT/NG/TV/MG Detection Kit Chlamydia trachomatis
Trichomonas vaginalis
Mycoplasma genitalium
Neisseria gonorrhoeae
ATWH HSV 1/2 Detection Kit Herpes Simplex Virus type 1
Herpes Simplex Virus type 2
ATWH Candidiasis Detection Kit Candida albicans
Candida dubliniensis
Candida tropicalis
Candida parapsilosis
Candida glabrata
Candida krusei
ATWH MH Detection Kit Mycoplasma Hominis
ATWH Ureaplasma Detection Kit Ureaplasma urealyticum
Ureaplasma parvum
ATWH Bacterial Vaginosis Detection Kit Gardnerella vaginalis
Atopobium vaginae
Bacterial Vaginosis Associated Bacteria-2
Megasphaera-1
Megasphaera-2
ATWH Internal Control Detection Kit Homo Sapiens