Mycoplasma pneumoniae PCR Detection Kit
The Atila Mycoplasma pneumoniae PCR Detection Kit is designed for rapid qualitative detection of M. pneumoniae DNA from sputum, oropharyngeal or nasopharyngeal swabs. The kit provides simple method to lyse bacterial cells and to release DNA; a PCR master mix which contains reagents and enzymes for PCR amplification; and a primer mix which are specific to the M. pneumoniae genome. In addition, the kit provides an internal control to identify possible PCR inhibition or sample preparation failure.
Mycoplasma pneumoniae is a bacterium that causes lung infection, or atypical pneumonia, especially in children and teenagers. M. pneumoniae in general causes mild illness and outbreaks tend to occur in closely related group of people. M. pneumoniae has been shown to be resistant to some antibiotics. And severe complications can occur that result in needing care or treatment in a hospital.
Features Include
Technical Specs
Workflow Overview for the Mycoplasma pneumoniae PCR Detection Kit
This section is for demonstrative purposes only and may be incomplete or inaccurate. Always refer to the product instructions for precise guidelines and directions.


Prepare the PCR reaction mix by combining 12.5μL x (N+2) of PCR master mix with 5μL x (N+2) of primer mix.

Finalize by adding H2O 5.5μL x (N+2) to complete the reaction mix.



Add 23μL of reaction mix per well along with 2μL of extracted DNA and internal control.

Perform PCR.

