Listeria monocytogenes PCR Detection Kit
This kit is designed for rapid qualitative detection of L. monocytogenes DNA isolated from food samples using a standard enrichment procedure.
Each kit includes 100 reactions.
This product is for research use only.
Listeria monocytogenes PCR Detection Kit
The Atila Listeria monocytogenes PCR Detection Kit is designed for rapid qualitative detection of L. monocytogenes DNA isolated from food samples using a standard enrichment procedure. The kit provides a PCR master mix which contains reagents and enzymes for PCR amplification; and a primer mix which are specific to the L. monocytogenes genome. In addition, the kit provides an internal control to identify possible PCR inhibition or sample preparation failure.
Listeria monocytogenes is one of the most virulent food borne bacterial pathogens and causes a serious illness listeriosis. The disease primarily affects older adults, pregnant women, newborns, and adults with weakened immune systems and has a 20-30% mortality rate.
The manifestations include septicemia, meningitis, encephalitis, corneal ulcer, pneumonia, and intrauterine or cervical infections in pregnant women, which may result in spontaneous abortion or stillbirth.
Features Include
Technical Specs
Workflow Overview for the Listeria monocytogenes PCR Detection Kit
This section is for demonstrative purposes only and may be incomplete or inaccurate. Always refer to the product instructions for precise guidelines and directions.
Prepare the PCR reaction mix by combining 12.5μL x (N+2) of PCR master mix with 5μL x (N+2) of primer mix.
Finalize by adding H2O 5.5μL x (N+2) to complete the reaction mix.
Add 23μL of reaction mix per well along with 2μL of extracted DNA and internal control.
Perform PCR.
