iAMP Sepsis Panel

The Atila iAMP Sepsis Panel is a real-time fluorescent isothermal PCR assay based on Atila’s proprietary isothermal amplification technology intended for the simultaneous qualitative detection and differentiation of nucleic acids from 30 common sepsis causing pathogens (summarized in Table 1) and for detection of 11 common drug-resistant gene markers (DR) (summarized in table 2) in plasma specimens obtained from individuals with or suspected of sepsis.

Each kit includes 96 tests.

This product is for research use only (RUO).

Category: SKU: iAMP-Sep-96

iAMP Sepsis Panel

Sepsis is a critical medical condition caused by the body’s overwhelming and dysregulated response to infection, often resulting in organ dysfunction and high mortality rates. One of the key challenges in sepsis management is the timely and accurate identification of the causative pathogen. Pathogen detection plays a pivotal role in guiding targeted antimicrobial therapy, which is essential for improving patient outcomes and combating the rise of antibiotic resistance.

Hence, identifying the pathogens associated with these infections, to receive appropriate treatment at an early stage is of utmost importance. To this end, Atila Biosystems aims to provide an easy to use, quick result, highly sensitive and specific detection assay. 

Our affordable iAMP Sepsis Panel uses a multiplexed isothermal PCR approach to identify 30 pathogens consisting of wide varieties of bacterial and fungal species, and 11 antimicrobial resistance gene markers in plasma or whole blood specimens. The assay uses a simple, extraction-free workflow to deliver results in under 90 minutes. The iAMP Sepsis Panel offers a vital combination of speed, accuracy, and comprehensiveness, enabling rapid identification of probable pathogens directly from plasma or whole blood.

 

Features Include

Fast Speed

From sample to answer is less than 90 minutes.

Simple Protocol

Simple, extraction-free workflow that saves time and money. The reagents are pre-loaded in the reaction tubes and sealed with gel. The only hands-on work is loading the samples.

Low Cost

Price per assay is much better than any other assay on the market for detection of a wide range of pathogens.

Flexibility

Our kits can be used for detection of any number of samples without wasting extra reagents.

Technical Specs

Reaction Time

Assay requires less than 1 hour of instrument time.

Sample Types

Plasma or whole blood.

Sensitivity

High sensitivity assay detects in a range of 10 copies per reaction.

Instruments

Compatible with iAMP Personal Station Real-Time PCR System, Atila PowerGene 9600 Plus Real-Time System, Bio-Rad CFX96, and other commonly used RT-qPCR instruments with 5 fluorescence channels.

Workflow Overview for the iAMP Sepsis Panel

This section is for demonstrative purposes only and may be incomplete or inaccurate. Always refer to the product instructions for precise guidelines and directions.

Dry swab in sample tube.
Mix 50uL plasma sample with 50uL 2xSPM
Incubate at room temperature for 2 minutes.
Add 10µL processed sample to well 1-8 of the strips
Run on standard PCR instrument.
Obtain results in 60 minutes

iAMP Sepsis Panel
Sample Types

  • Urine specimens processed and tested with iAMP UISoluton Assay.
  • Direct urine specimens without any preservatives.Note: Clean-catch urine specimens are highly recommended.
  • Direct dry wound swab specimens collected by clinicians with Atila Swab Sample Collection Device (iAMP-SWA-SCD). Alternatively, specimens collected with sample collection devices from other vendors may be used after self-validation.
  • Wound swab specimens collected in transport medium (with sample collection devices from other vendors) may be used after self-validation.

iAMP Sepsis Panel
Sample Types

  • Vaginal/cervical swab collected as dry swabs or in 1X iAMP Sample Buffer.
  • Vaginal/cervical swab collected in commonly used transport medium.
  • First-void urine specimen without any preservatives.

iAMP Sepsis Panel
Sample Types

  • Vaginal/cervical swab collected as dry swabs or in 1X iAMP Sample Buffer, or in commonly used transport medium.
  • First-void urine specimen without any preservatives.
  • Ocular swab collected as dry swabs or in 1X iAMP Sample Buffer, or in commonly used transport medium.

iAMP Sepsis Panel
Assay Targets

  • Trichophyton spp.
  • Aspergillus
  • Curvularia spp.
  • Fusarium solani
  • Epidermophyton floccosum
  • Cryptococcus
  • Malassezia spp.
  • Sarocladium strictum
  • Scopulariopsis brevicaulis
  • Microsporum audouinii/canis
  • Alternaria
  • NeoScytalidim d/h
  • Geotrichum candidum
  • Candida spp.
  • Trichosporon
  • Neofusicoccum mangiferae

iAMP UISolution Assay for UTIs
Assay Targets

  • Acinetobacter baumannii
  • Citrobacter spp.
  • Enterobacter cloacae
  • Enterococcus spp.
  • Escherichia coli
  • Klebsiella aerogenes
  • Klebsiella oxytoca
  • Klebsiella pneumoniae
  • Morganella morganii
  • Proteus spp.
  • Providencia stuartii
  • Pseudomonas aeruginosa
  • Staphylococcus saprophyticus
  • Streptococcus agalactiae
  • Candida albicans
Pathogen TypeSpecies
BacteriaAcinetobacter baumannii
Anaerococcus spp
Bacteroides fragilis
Citrobacter spp.
Enterococcus spp.
Escherichia coli
Klebsiella oxytoca
Klebsiella pneumoniae
Proteus spp.
Pseudomonas aeruginosa
Serratia marcescens
Staphylococcus aureus
Staphylococcus epidermidis
Streptococcus agalactiae
Streptococcus pyogenes
VirusHerpes simplex virus 1
FungiCandida spp.
Candida glabrata
Candida krusei

iAMP Sepsis Panel
Assay Targets

  • Respiratory syncytial virus
  • Influenza A
  • Influenza B
  • SARS-CoV-2
  • Rhinovirus
  • Enterovirus
  • Coronavirus (229E, HKU1, OC43, NL63)
  • Parainfluenza virus (1-4)
  • Human adenovirus
  • Human bocavirus
  • Human metapneumovirus
  • Mycoplasma pneumonia
  • Klebsiella pneumonia
  • Bordetella parapertussis
  • Bordetella pertussis
  • Streptococcus pneumonia
  • Haemophilus influenzae
  • Legionella pneumophila
  • Chlamydia pneumonia
Detection Kit Species
ATWH CT/NG/TV/MG Detection Kit Chlamydia trachomatis
Trichomonas vaginalis
Mycoplasma genitalium
Neisseria gonorrhoeae
ATWH HSV 1/2 Detection Kit Herpes Simplex Virus type 1
Herpes Simplex Virus type 2
ATWH Candidiasis Detection Kit Candida albicans
Candida dubliniensis
Candida tropicalis
Candida parapsilosis
Candida glabrata
Candida krusei
ATWH MH Detection Kit Mycoplasma Hominis
ATWH Ureaplasma Detection Kit Ureaplasma urealyticum
Ureaplasma parvum
ATWH Bacterial Vaginosis Detection Kit Gardnerella vaginalis
Atopobium vaginae
Bacterial Vaginosis Associated Bacteria-2
Megasphaera-1
Megasphaera-2
ATWH Internal Control Detection Kit Homo Sapiens