iAMP NG Resistance Assay
The iAMP NG Resistance Assay is a real-time fluorescent isothermal PCR assay based on Atila’s proprietary isothermal amplification technology intended for the qualitative detection of gyrA S91F (mutant) markers that are associated with susceptibility or resistance to the fluoroquinolone antibiotic, ciprofloxacin from males that are identified as Neisseria gonorrhoea (NG) positive by iAMP CT/NG/TV/MG Detection kit.
Each kit includes 100 tests.
This product is CE-IVD certified. It is for research use only in US.
iAMP NG Resistance Assay
The iAMP NG Resistance Assay is a real-time fluorescent isothermal PCR assay based on Atila’s proprietary isothermal amplification technology intended for the qualitative detection of gyrA S91F (mutant) markers that are associated with susceptibility or resistance to the fluoroquinolone antibiotic, ciprofloxacin in clinician-collected or self-collected vaginal swab samples, female or male first-void urine samples (defined as the first 20-30 mL of the urine stream), clinician-collected endocervical swabs from females or clinician-collected urethral swabs from males that are identified as Neisseria gonorrhea (NG) positive by the iAMP CT/NG/TV/MG Detection Kit.
Gonorrhoea is a sexually transmitted infection (STI) that remains a major public health concern. Over the past 80 years, many antimicrobial medicines were used to fight against Gonorrhoea, such as tetracyclines, macrolides, sulphonamides, trimethoprim combinations, and more recently, quinolones and ciprofloxacin. The overuse of these medicines results in antimicrobial resistance in Neisseria gonorrhoeae.
In many countries, ciprofloxacin resistance is extremely high, which makes the treatment of gonorrhoea infection difficult.
Features Include
Technical Specs
Workflow Overview A
Workflow Overview for the iAMP NG Resistance Assay
This section is for demonstrative purposes only and may be incomplete or inaccurate. Always refer to the product instructions for precise guidelines and directions.
Left over sample of NG positive sample.
Thaw at room temperature for 10 minutes.
Vortex.
Add 20 µL reaction mix and 5 µL processed sample.
Run on standard PCR instrument.
From sample to answer in less than 90 minutes.
Workflow Overview B
Workflow Overview for the iAMP NG Resistance Assay
This section is for demonstrative purposes only and may be incomplete or inaccurate. Always refer to the product instructions for precise guidelines and directions.
Centrifuge at 10,000 RPM for 15 min, remove the supernatant completely.
Add 120 µL Atila 1x sample buffer and vortex thoroughly.
Incubate at 95C for 10 minutes.
Add 20 µL reaction mix and 5 µL processed sample.
Run on standard PCR instrument.
From sample to answer in less than 90 minutes.
Workflow Overview C
Workflow Overview for the iAMP NG Resistance Assay
This section is for demonstrative purposes only and may be incomplete or inaccurate. Always refer to the product instructions for precise guidelines and directions.
Dry swab in sample tube.
Add up to 750µL Atila 1x sample buffer.
Vortex and incubate at 95C for 10 minutes.
Add 20 µL reaction mix and 5 µL processed sample.
Run on standard PCR instrument.