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iAMP® Gastrointestinal Panel

The iAMP® Gastrointestinal Panel is an extraction-free, real-time fluorescent iso qPCR assay based on Atila’s proprietary amplification technology intended for the simultaneous qualitative detection and differentiation of nucleic acids from multiple bacteria, parasites, and viruses (summarized in Table 1). To be used directly on stool samples obtained from individuals with or suspected of gastrointestinal infection.

Positive results do not rule out co-infection with organisms not included in the iAMP®  Gastrointestinal Panel. Negative results do not preclude infection with bacterial, viral or other parasitic pathogens that are not listed in the iAMP® Gastrointestinal Panel. The iAMP® Gastrointestinal Panel is intended for use by trained laboratory personnel specifically instructed and trained in the techniques of real-time nucleic acid amplification.

Each kit includes 100 tests.

This product is for research use only (RUO).

Category: SKU: iAMP-GI-100

iAMP® Gastrointestinal Panel

Gastrointestinal (GI) infections are among the most common illnesses worldwide, affecting millions of people every year and placing a significant burden on healthcare systems. These infections are often transmitted through contaminated food, water, or close contact. Many GI pathogens produce symptoms, such as diarrhea, abdominal pain, nausea, and fever, like other conditions making diagnosis complicated. Delays in diagnosis may lead to worsening symptoms and potentially escalating to life-threatening conditions or long-term issues.

The iAMP® Gastrointestinal Panel is a rapid, real-time fluorescent iso qPCR panel that simultaneously detects 8 bacterial pathogens, 5 viral pathogens, 4 parasitic pathogens, and 6 diarrheagenic E.Coli/Shigella pathogens directly from stool samples without nucleic acid extraction. This panel aids in the diagnosis of pathogen(s) causing gastrointestinal illness, in conjunction with other clinical, laboratory, and epidemiological information.

Features Include

Fast Speed

Assay requires 1 hour of instrument time.

Easy to Use

Extraction-free workflow that saves time and money.

Low Cost

Price per assay is more affordable than any other GI assay on the market.

Flexibility

Our kits can be used for detection of any number of samples without wasting extra reagents.

Technical Specs

Sample Types

Stool specimens.

Sensitivity

High sensitivity assay.

Instruments

Compatible with iAMP Personal Station Real-Time PCR System, Atila PowerGene 9600 Plus Real-Time System, Bio-Rad CFX96, and other commonly used RT-qPCR instruments with FAM/HEX/ROX/CY5 fluorescence channels.

Workflow Overview for the iAMP® Gastrointestinal Panel

This section is for demonstrative purposes only and may be incomplete or inaccurate. Always refer to the product instructions for precise guidelines and directions.

Take ~30 mg stool sample.
Add 1mL sample processing buffer, vortex for 1 minute.
Incubate at 95°C for 10 minutes.
Quick spin for 1 minute, load 5 uL supernatant into the reaction.
Run on standard PCR instrument.
Obtain results in 60 minutes.

iAMP® Gastrointestinal Panel
Sample Types

  • Urine specimens processed and tested with iAMP UISoluton Assay.
  • Direct urine specimens without any preservatives.Note: Clean-catch urine specimens are highly recommended.
  • Direct dry wound swab specimens collected by clinicians with Atila Swab Sample Collection Device (iAMP-SWA-SCD). Alternatively, specimens collected with sample collection devices from other vendors may be used after self-validation.
  • Wound swab specimens collected in transport medium (with sample collection devices from other vendors) may be used after self-validation.

iAMP® Gastrointestinal Panel
Sample Types

  • Vaginal/cervical swab collected as dry swabs or in 1X iAMP Sample Buffer.
  • Vaginal/cervical swab collected in commonly used transport medium.
  • First-void urine specimen without any preservatives.

iAMP® Gastrointestinal Panel
Sample Types

  • Vaginal/cervical swab collected as dry swabs or in 1X iAMP Sample Buffer, or in commonly used transport medium.
  • First-void urine specimen without any preservatives.
  • Ocular swab collected as dry swabs or in 1X iAMP Sample Buffer, or in commonly used transport medium.

iAMP® Gastrointestinal Panel
Assay Targets

  • Trichophyton spp.
  • Aspergillus
  • Curvularia spp.
  • Fusarium solani
  • Epidermophyton floccosum
  • Cryptococcus
  • Malassezia spp.
  • Sarocladium strictum
  • Scopulariopsis brevicaulis
  • Microsporum audouinii/canis
  • Alternaria
  • NeoScytalidim d/h
  • Geotrichum candidum
  • Candida spp.
  • Trichosporon
  • Neofusicoccum mangiferae

iAMP UISolution Assay for UTIs
Assay Targets

  • Acinetobacter baumannii
  • Citrobacter spp.
  • Enterobacter cloacae
  • Enterococcus spp.
  • Escherichia coli
  • Klebsiella aerogenes
  • Klebsiella oxytoca
  • Klebsiella pneumoniae
  • Morganella morganii
  • Proteus spp.
  • Providencia stuartii
  • Pseudomonas aeruginosa
  • Staphylococcus saprophyticus
  • Streptococcus agalactiae
  • Candida albicans
Pathogen TypeSpecies
BacteriaAcinetobacter baumannii
Anaerococcus spp
Bacteroides fragilis
Citrobacter spp.
Enterococcus spp.
Escherichia coli
Klebsiella oxytoca
Klebsiella pneumoniae
Proteus spp.
Pseudomonas aeruginosa
Serratia marcescens
Staphylococcus aureus
Staphylococcus epidermidis
Streptococcus agalactiae
Streptococcus pyogenes
VirusHerpes simplex virus 1
FungiCandida spp.
Candida glabrata
Candida krusei

iAMP® Gastrointestinal Panel
Assay Targets

  • Respiratory syncytial virus
  • Influenza A
  • Influenza B
  • SARS-CoV-2
  • Rhinovirus
  • Enterovirus
  • Coronavirus (229E, HKU1, OC43, NL63)
  • Parainfluenza virus (1-4)
  • Human adenovirus
  • Human bocavirus
  • Human metapneumovirus
  • Mycoplasma pneumonia
  • Klebsiella pneumonia
  • Bordetella parapertussis
  • Bordetella pertussis
  • Streptococcus pneumonia
  • Haemophilus influenzae
  • Legionella pneumophila
  • Chlamydia pneumonia
Detection Kit Species
ATWH CT/NG/TV/MG Detection Kit Chlamydia trachomatis
Trichomonas vaginalis
Mycoplasma genitalium
Neisseria gonorrhoeae
ATWH HSV 1/2 Detection Kit Herpes Simplex Virus type 1
Herpes Simplex Virus type 2
ATWH Candidiasis Detection Kit Candida albicans
Candida dubliniensis
Candida tropicalis
Candida parapsilosis
Candida glabrata
Candida krusei
ATWH MH Detection Kit Mycoplasma Hominis
ATWH Ureaplasma Detection Kit Ureaplasma urealyticum
Ureaplasma parvum
ATWH Bacterial Vaginosis Detection Kit Gardnerella vaginalis
Atopobium vaginae
Bacterial Vaginosis Associated Bacteria-2
Megasphaera-1
Megasphaera-2
ATWH Internal Control Detection Kit Homo Sapiens