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Hotspot Cancer Panel Sequencing Library Preparation Kit

This kit targets amplicon regions of cancer related genes and provides superior sensitivity for detection of cancer mutations. The resulting sample library is compatible with Illumina NGS sequencing.

Each kit includes 100 reactions.

This product is for research use only.

Categories: , SKU: ACPN-HT-100

Hotspot Cancer Panel Sequencing Library Preparation Kit

The Atila Hotspot Cancer Panel Sequencing Library Preparation Kit targets amplicon regions of more than 50 cancer related genes, including BRAF, KRAS, and EGFR etc, and provides superior sensitivity for detection of more than 2700 cancer mutation hotspots.

The resulting sample library is compatible with Illumina NGS sequencing. The current method employs novel primer design strategies, greatly simplifies sample preparation procedures, and enables rapid screening and discovering genetic variations.

Cancer hotspots are commonly observed mutations in known oncogenes and tumor suppressor genes across different cancer types.

Cancer is a type of diseases caused by accumulation of genetic changes, which results in abnormal cell growth and invasive behaviors that potentially spread to other body parts. Most common nucleotide sequence changes in cancer involve single-nucleotide variants and small insertions or deletions.

Features Include

High Sensitivity

The kit can use as low as 100pg purified DNA.

Simple Procedures

Hands-on time is as short as 30 minutes.

Short Preparation Time

Sample library is ready within 2.5 to 3 hours.

High Specificity

High on target rate and high uniformity.

Technical Specs

Assay Targets

ABL1, EGFR, GNAQ, KRAS, PTPN11, AKT1, ERBB2, GNAS, MET, RB1, ALK, ERBB4, HNF1A, MLH1, RET, APC, EZH2, HRAS, MPL, SMAD4, ATM, FBXW7, IDH1, NOTCH1, SMARCB1, BRAF, FGFR1, IDH2, NPM1, SMO, CDH1, FGFR2, JAK2, NRAS, SRC, CDKN2A, FGFR3, JAK3, PDGFRA, STK11, CSF1R, FLT3, KDR, PIK3CA, TP53, CTNNB1, GNA11, KIT, PTEN and VHL etc.

Reaction Time

2.5 to 3 hours.

Sample Types

Purified DNA, cell free DNA & FFPE DNA.

Sensitivity

The kit can use as low as 100pg purified DNA.

Instruments

Compatible with MiniSeq and MiSeq instruments.

Workflow Overview for the Hotspot Cancer Panel Sequencing Library Preparation Kit

This section is for demonstrative purposes only and may be incomplete or inaccurate. Always refer to the product instructions for precise guidelines and directions.

Illumina sequencing.
Obtain result.
SIMPLE. FAST. AFFORDABLE.

Please contact us for a quote or any questions about our iAMP Automatic Instrument.

Hotspot Cancer Panel Sequencing Library Preparation Kit
Sample Types

  • Urine specimens processed and tested with iAMP UISoluton Assay.
  • Direct urine specimens without any preservatives.Note: Clean-catch urine specimens are highly recommended.
  • Direct dry wound swab specimens collected by clinicians with Atila Swab Sample Collection Device (iAMP-SWA-SCD). Alternatively, specimens collected with sample collection devices from other vendors may be used after self-validation.
  • Wound swab specimens collected in transport medium (with sample collection devices from other vendors) may be used after self-validation.

Hotspot Cancer Panel Sequencing Library Preparation Kit
Sample Types

  • Vaginal/cervical swab collected as dry swabs or in 1X iAMP Sample Buffer.
  • Vaginal/cervical swab collected in commonly used transport medium.
  • First-void urine specimen without any preservatives.

Hotspot Cancer Panel Sequencing Library Preparation Kit
Sample Types

  • Vaginal/cervical swab collected as dry swabs or in 1X iAMP Sample Buffer, or in commonly used transport medium.
  • First-void urine specimen without any preservatives.
  • Ocular swab collected as dry swabs or in 1X iAMP Sample Buffer, or in commonly used transport medium.

Hotspot Cancer Panel Sequencing Library Preparation Kit
Assay Targets

  • Trichophyton spp.
  • Aspergillus
  • Curvularia spp.
  • Fusarium solani
  • Epidermophyton floccosum
  • Cryptococcus
  • Malassezia spp.
  • Sarocladium strictum
  • Scopulariopsis brevicaulis
  • Microsporum audouinii/canis
  • Alternaria
  • NeoScytalidim d/h
  • Geotrichum candidum
  • Candida spp.
  • Trichosporon
  • Neofusicoccum mangiferae

iAMP UISolution Assay for UTIs
Assay Targets

  • Acinetobacter baumannii
  • Citrobacter spp.
  • Enterobacter cloacae
  • Enterococcus spp.
  • Escherichia coli
  • Klebsiella aerogenes
  • Klebsiella oxytoca
  • Klebsiella pneumoniae
  • Morganella morganii
  • Proteus spp.
  • Providencia stuartii
  • Pseudomonas aeruginosa
  • Staphylococcus saprophyticus
  • Streptococcus agalactiae
  • Candida albicans
Pathogen TypeSpecies
BacteriaAcinetobacter baumannii
Anaerococcus spp
Bacteroides fragilis
Citrobacter spp.
Enterococcus spp.
Escherichia coli
Klebsiella oxytoca
Klebsiella pneumoniae
Proteus spp.
Pseudomonas aeruginosa
Serratia marcescens
Staphylococcus aureus
Staphylococcus epidermidis
Streptococcus agalactiae
Streptococcus pyogenes
VirusHerpes simplex virus 1
FungiCandida spp.
Candida glabrata
Candida krusei

Hotspot Cancer Panel Sequencing Library Preparation Kit
Assay Targets

  • Respiratory syncytial virus
  • Influenza A
  • Influenza B
  • SARS-CoV-2
  • Rhinovirus
  • Enterovirus
  • Coronavirus (229E, HKU1, OC43, NL63)
  • Parainfluenza virus (1-4)
  • Human adenovirus
  • Human bocavirus
  • Human metapneumovirus
  • Mycoplasma pneumonia
  • Klebsiella pneumonia
  • Bordetella parapertussis
  • Bordetella pertussis
  • Streptococcus pneumonia
  • Haemophilus influenzae
  • Legionella pneumophila
  • Chlamydia pneumonia
Detection Kit Species
ATWH CT/NG/TV/MG Detection Kit Chlamydia trachomatis
Trichomonas vaginalis
Mycoplasma genitalium
Neisseria gonorrhoeae
ATWH HSV 1/2 Detection Kit Herpes Simplex Virus type 1
Herpes Simplex Virus type 2
ATWH Candidiasis Detection Kit Candida albicans
Candida dubliniensis
Candida tropicalis
Candida parapsilosis
Candida glabrata
Candida krusei
ATWH MH Detection Kit Mycoplasma Hominis
ATWH Ureaplasma Detection Kit Ureaplasma urealyticum
Ureaplasma parvum
ATWH Bacterial Vaginosis Detection Kit Gardnerella vaginalis
Atopobium vaginae
Bacterial Vaginosis Associated Bacteria-2
Megasphaera-1
Megasphaera-2
ATWH Internal Control Detection Kit Homo Sapiens