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Atila Gender Determination Kit

The Atila Gender Determination Kit is a real-time nucleic acid amplification assay for the in vitro detection  of Y chromosome gene from cell-free fetal DNA circulating in the mother’s bloodstream during pregnancy.  This test can be used to determine the baby’s gender from as early as 6-8 weeks of gestation. The presence  of Y chromosome gene indicates a male baby, while the absence of Y chromosome gene indicates a female  baby. It is not intended for general health screening or for use as the sole basis of diagnosis without clinical correlation. 

The Atila Gender Determination Kit is intended for use by qualified laboratory professionals who have  been specifically trained in real-time nucleic acid amplification techniques. 

Each kit includes 100 tests.

This product is for research use only (RUO).

Category: SKU: qGD-100-RUO

Atila Gender Determination Kit

The discovery of cell-free fetal DNA from the mother’s plasma during pregnancy has made it possible to  determine the baby’s gender without using invasive methods such as chorionic villus sampling or relying  on ultrasonography which cannot be done in the first trimester. The concentration of cell-free fetal DNA  in maternal plasma corresponds to 3.4% (range 0.39%–11.9%) and 6.2% (range 2.33%–11.4%) of the total plasma DNA in early and late pregnancy respectively, making real-time PCR using cell-free fetal DNA a reliable noninvasive method for fetal gender determination. 

The Atila Gender Determination Kit is a multiplex real-time polymerase chain reaction (PCR) assay based  on proprietary technology designed to improve the accuracy and efficiency of fetal gender determination.  Multiple sets of oligonucleotides are specifically designed to detect Y chromosome genes as well as an  internal control gene. The presence of the Y chromosome gene in maternal plasma cell-free DNA indicates  a male baby, while the absence indicates a female baby. 

Features Include

Fast Speed

From DNA to detection in less than 90-120 minutes.

Easy to Use

Simple workflow that saves time and money.

Low Cost

Price per assay is more affordable than any other assay on the market for gender determination.

Flexibility

Our kits can be used for detection of any number of samples without wasting extra reagents.

Technical Specs

Reaction Time

Assay requires less than 1 hour of instrument time.

Sample Types

Purified cfDNA from maternal blood specimens.

Sensitivity

High sensitivity assay.

Instruments

Compatible Bio-Rad CFX96, Atila Powergene, and other commonly used RT-qPCR instruments with 2 fluorescence channels.

Workflow Overview for the Atila Gender Determination Kit

This section is for demonstrative purposes only and may be incomplete or inaccurate. Always refer to the product instructions for precise guidelines and directions.

Master Mix Preparation
Reaction Setup
Run on Atila PowerGene or Bio-Rad CFX96
Obtain result report in less than 60 minutes.

Atila Gender Determination Kit
Sample Types

  • Urine specimens processed and tested with iAMP UISoluton Assay.
  • Direct urine specimens without any preservatives.Note: Clean-catch urine specimens are highly recommended.
  • Direct dry wound swab specimens collected by clinicians with Atila Swab Sample Collection Device (iAMP-SWA-SCD). Alternatively, specimens collected with sample collection devices from other vendors may be used after self-validation.
  • Wound swab specimens collected in transport medium (with sample collection devices from other vendors) may be used after self-validation.

Atila Gender Determination Kit
Sample Types

  • Vaginal/cervical swab collected as dry swabs or in 1X iAMP Sample Buffer.
  • Vaginal/cervical swab collected in commonly used transport medium.
  • First-void urine specimen without any preservatives.

Atila Gender Determination Kit
Sample Types

  • Vaginal/cervical swab collected as dry swabs or in 1X iAMP Sample Buffer, or in commonly used transport medium.
  • First-void urine specimen without any preservatives.
  • Ocular swab collected as dry swabs or in 1X iAMP Sample Buffer, or in commonly used transport medium.

Atila Gender Determination Kit
Assay Targets

  • Trichophyton spp.
  • Aspergillus
  • Curvularia spp.
  • Fusarium solani
  • Epidermophyton floccosum
  • Cryptococcus
  • Malassezia spp.
  • Sarocladium strictum
  • Scopulariopsis brevicaulis
  • Microsporum audouinii/canis
  • Alternaria
  • NeoScytalidim d/h
  • Geotrichum candidum
  • Candida spp.
  • Trichosporon
  • Neofusicoccum mangiferae

iAMP UISolution Assay for UTIs
Assay Targets

  • Acinetobacter baumannii
  • Citrobacter spp.
  • Enterobacter cloacae
  • Enterococcus spp.
  • Escherichia coli
  • Klebsiella aerogenes
  • Klebsiella oxytoca
  • Klebsiella pneumoniae
  • Morganella morganii
  • Proteus spp.
  • Providencia stuartii
  • Pseudomonas aeruginosa
  • Staphylococcus saprophyticus
  • Streptococcus agalactiae
  • Candida albicans
Pathogen TypeSpecies
BacteriaAcinetobacter baumannii
Anaerococcus spp
Bacteroides fragilis
Citrobacter spp.
Enterococcus spp.
Escherichia coli
Klebsiella oxytoca
Klebsiella pneumoniae
Proteus spp.
Pseudomonas aeruginosa
Serratia marcescens
Staphylococcus aureus
Staphylococcus epidermidis
Streptococcus agalactiae
Streptococcus pyogenes
VirusHerpes simplex virus 1
FungiCandida spp.
Candida glabrata
Candida krusei

Atila Gender Determination Kit
Assay Targets

  • Respiratory syncytial virus
  • Influenza A
  • Influenza B
  • SARS-CoV-2
  • Rhinovirus
  • Enterovirus
  • Coronavirus (229E, HKU1, OC43, NL63)
  • Parainfluenza virus (1-4)
  • Human adenovirus
  • Human bocavirus
  • Human metapneumovirus
  • Mycoplasma pneumonia
  • Klebsiella pneumonia
  • Bordetella parapertussis
  • Bordetella pertussis
  • Streptococcus pneumonia
  • Haemophilus influenzae
  • Legionella pneumophila
  • Chlamydia pneumonia
Detection Kit Species
ATWH CT/NG/TV/MG Detection Kit Chlamydia trachomatis
Trichomonas vaginalis
Mycoplasma genitalium
Neisseria gonorrhoeae
ATWH HSV 1/2 Detection Kit Herpes Simplex Virus type 1
Herpes Simplex Virus type 2
ATWH Candidiasis Detection Kit Candida albicans
Candida dubliniensis
Candida tropicalis
Candida parapsilosis
Candida glabrata
Candida krusei
ATWH MH Detection Kit Mycoplasma Hominis
ATWH Ureaplasma Detection Kit Ureaplasma urealyticum
Ureaplasma parvum
ATWH Bacterial Vaginosis Detection Kit Gardnerella vaginalis
Atopobium vaginae
Bacterial Vaginosis Associated Bacteria-2
Megasphaera-1
Megasphaera-2
ATWH Internal Control Detection Kit Homo Sapiens