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AmpFire Malaria Detection Assay

The AmpFire Malaria Detection Assay kit is an in vitro nucleic acid isothermal amplification with real time fluorescence detection assay for the qualitative detection of DNA from Plasmodium falciparum, Plasmodium knowlesi ,Plasmodium malariae, Plasmodium ovale and Plasmodium vivax and differentiate Plasmodium falciparum, the most common pathogen from other species from different human sample types.

Each kit includes 100 tests.

This product is RUO (for research only).

Category: SKU: MAL-100-RUO

AmpFire Malaria Detection Assay

Malaria is a serious and sometimes fatal disease caused by a parasite that commonly infects a certain type of mosquito which feeds on humans. People who get malaria are typically very sick with high fevers, shaking chills, and flu-like illness.

Four kinds of malaria parasites infect humans: Plasmodium falciparum, P. vivax, P. ovale, and P. malariae. In addition, P. knowlesi, a type of malaria that naturally infects macaques in Southeast Asia, also infects humans, causing malaria that is transmitted from animal to human (“zoonotic” malaria). P. falciparum is the type of malaria that is most likely to result in severe infections and if not promptly treated, may lead to death. Although malaria can be a deadly disease, illness and death from malaria can usually be prevented.

About 2,000 cases of malaria are diagnosed in the United States each year. The vast majority of cases in the United States are in travelers and immigrants returning from parts of the world where malaria transmission occurs, including sub-Saharan Africa and South Asia.

Globally, the World Health Organization estimates that in 2020, 241 million clinical cases of malaria occurred, and 627,000 people died of malaria, most of them children in Africa. Because malaria causes so much illness and death, the disease is a great drain on many national economies. Since many countries with malaria are already among the poorer nations, the disease maintains a vicious cycle of disease and poverty.

The Atila AmpFire Malaria Assay, is an isothermal nucleic acid amplification assay for the qualitative detection of Plasmodium falciparum, P. vivax, P. ovale, P. malariae and P. knowlesi. Specific primers and fluorescent probes are used to amplify regions of pathogen genomic DNA under isothermal conditions. The assay detects Plasmodium falciparum, P. vivax, P. ovale, P. malariae and P. knowlesi and allows simultaneous identification of the Plasmodium falciparum.

Features Include

Fast Speed

From sample to answer is less than 90 minutes. Hands on times are less than 15 minutes per assay.

Simple Protocol

Simple, extraction-free workflow that saves time and money.

Low Cost

Price per assay is much better than any other kits on the market for Malaria detection.

Flexibility

Our kits can be used for detection of any number of samples without wasting extra reagents.

Technical Specs

Assay Targets

Plasmodium falciparum, P. vivax, P. ovale, P. malariae and P. knowlesi genomic DNA, human genomic DNA

Reaction Time

Assay requires 1 hour of instrument time.

Sensitivity

High sensitivity assay detects as low as 20 copies of pathogen genome per reaction.

Instruments

Compatible with Bio-Rad CFX96, ABI7500, Quant-Studio systems, Powergene9600, iAMP-PS96 , and other commonly used qPCR instruments.

Workflow Overview for the AmpFire Malaria Detection Assay

This section is for demonstrative purposes only and may be incomplete or inaccurate. Always refer to the product instructions for precise guidelines and directions.

Clinical serum sample in Atila sample lysis buffer.
Transfer 5µL to plate or strip. Add 20µL Reaction Master Mix.
Add 20µL Reaction Master Mix.
From sample to answer in less than 90 minutes.
SIMPLE. FAST. AFFORDABLE.

Please contact us for a quote or any questions about our iAMP Automatic Instrument.

AmpFire Malaria Detection Assay
Sample Types

  • Urine specimens processed and tested with iAMP UISoluton Assay.
  • Direct urine specimens without any preservatives.Note: Clean-catch urine specimens are highly recommended.
  • Direct dry wound swab specimens collected by clinicians with Atila Swab Sample Collection Device (iAMP-SWA-SCD). Alternatively, specimens collected with sample collection devices from other vendors may be used after self-validation.
  • Wound swab specimens collected in transport medium (with sample collection devices from other vendors) may be used after self-validation.

AmpFire Malaria Detection Assay
Sample Types

  • Vaginal/cervical swab collected as dry swabs or in 1X iAMP Sample Buffer.
  • Vaginal/cervical swab collected in commonly used transport medium.
  • First-void urine specimen without any preservatives.

AmpFire Malaria Detection Assay
Sample Types

  • Vaginal/cervical swab collected as dry swabs or in 1X iAMP Sample Buffer, or in commonly used transport medium.
  • First-void urine specimen without any preservatives.
  • Ocular swab collected as dry swabs or in 1X iAMP Sample Buffer, or in commonly used transport medium.

AmpFire Malaria Detection Assay
Assay Targets

  • Trichophyton spp.
  • Aspergillus
  • Curvularia spp.
  • Fusarium solani
  • Epidermophyton floccosum
  • Cryptococcus
  • Malassezia spp.
  • Sarocladium strictum
  • Scopulariopsis brevicaulis
  • Microsporum audouinii/canis
  • Alternaria
  • NeoScytalidim d/h
  • Geotrichum candidum
  • Candida spp.
  • Trichosporon
  • Neofusicoccum mangiferae

iAMP UISolution Assay for UTIs
Assay Targets

  • Acinetobacter baumannii
  • Citrobacter spp.
  • Enterobacter cloacae
  • Enterococcus spp.
  • Escherichia coli
  • Klebsiella aerogenes
  • Klebsiella oxytoca
  • Klebsiella pneumoniae
  • Morganella morganii
  • Proteus spp.
  • Providencia stuartii
  • Pseudomonas aeruginosa
  • Staphylococcus saprophyticus
  • Streptococcus agalactiae
  • Candida albicans
Pathogen TypeSpecies
BacteriaAcinetobacter baumannii
Anaerococcus spp
Bacteroides fragilis
Citrobacter spp.
Enterococcus spp.
Escherichia coli
Klebsiella oxytoca
Klebsiella pneumoniae
Proteus spp.
Pseudomonas aeruginosa
Serratia marcescens
Staphylococcus aureus
Staphylococcus epidermidis
Streptococcus agalactiae
Streptococcus pyogenes
VirusHerpes simplex virus 1
FungiCandida spp.
Candida glabrata
Candida krusei

AmpFire Malaria Detection Assay
Assay Targets

  • Respiratory syncytial virus
  • Influenza A
  • Influenza B
  • SARS-CoV-2
  • Rhinovirus
  • Enterovirus
  • Coronavirus (229E, HKU1, OC43, NL63)
  • Parainfluenza virus (1-4)
  • Human adenovirus
  • Human bocavirus
  • Human metapneumovirus
  • Mycoplasma pneumonia
  • Klebsiella pneumonia
  • Bordetella parapertussis
  • Bordetella pertussis
  • Streptococcus pneumonia
  • Haemophilus influenzae
  • Legionella pneumophila
  • Chlamydia pneumonia
Detection Kit Species
ATWH CT/NG/TV/MG Detection Kit Chlamydia trachomatis
Trichomonas vaginalis
Mycoplasma genitalium
Neisseria gonorrhoeae
ATWH HSV 1/2 Detection Kit Herpes Simplex Virus type 1
Herpes Simplex Virus type 2
ATWH Candidiasis Detection Kit Candida albicans
Candida dubliniensis
Candida tropicalis
Candida parapsilosis
Candida glabrata
Candida krusei
ATWH MH Detection Kit Mycoplasma Hominis
ATWH Ureaplasma Detection Kit Ureaplasma urealyticum
Ureaplasma parvum
ATWH Bacterial Vaginosis Detection Kit Gardnerella vaginalis
Atopobium vaginae
Bacterial Vaginosis Associated Bacteria-2
Megasphaera-1
Megasphaera-2
ATWH Internal Control Detection Kit Homo Sapiens