HotSpot Cancer Panel NGS Library Preparation Kit


High multiplex PCR based library preparation. No ligation involved. Fast and simple.

Description:

Cancer is a type of diseases caused by accumulation of genetic changes, which results in abnormal cell growth and invasive behaviors that potentially spread to other body parts. Most common nucleotide sequence changes in cancer involve single-nucleotide variants and small insertions or deletions.

Cancer hotspots are commonly observed mutations in known oncogenes and tumor suppressor genes across different cancer types. Atila Hotspot Cancer Panel NGS library preparation Kit targets 195 amplicon regions of 50 cancer related genes, including BRAF, KRAS, and EGFR etc, and provides superior sensitivity for detection of more than 2700 cancer mutation hotspots. The resulting sample library is compatible with Illumina NGS sequencing. The current method employs novel primer design strategies, greatly simplifies sample preparation procedures, and enables rapid screening and discovering genetic variations.

Breast cancer is a type of cancer that occurs when cells in breast mutate and keep reproducing. It is one of the most common cancer types and one of the leading causes of cancer death among women. About 12% women in the United States will develop breast cancer in her lifetime. Atila Breast Cancer Panel NGS library preparation Kit targets 139 amplicon regions of 16 breast cancer related genes, including AKT1, BAP1, BRCA1, BRCA2, BRIP1, CCND1, CHEK2, EP300, ERBB2, GATA3, MAP2K4, PALB2, PBRM1, PIK3CA, RB1, and TP53 etc. The resulting sample library is compatible with Illumina NGS sequencing. The current method employs novel primer design strategies, greatly simplifies sample preparation procedures, and enables rapid screening and discovering genetic variations.

Lung cancer is one of the lead causes of cancer death in the United States and worldwide. The two main types of lung cancer are small-cell lung carcinoma (SCLC) and non-small-cell lung carcinoma (NSCLC). Atila Lung Cancer Panel NGS library preparation Kit targets 138 amplicon regions of 24 lung cancer related genes, including AKT1, ALK, BAP1, BRAF, CCDC6, EGFR, EML4, ERBB2, FGFR2, KDR, KRAS, LRIG3, MAP2K1, MAP2K2, MYCL1, NFE2L2, RB1, RET, ROS1, SLC34A2, SMARCA4, STK11, TFG, and TP53 etc. The resulting sample library is compatible with Illumina NGS sequencing. The current method employs novel primer design strategies, greatly simplifies sample preparation procedures, and enables rapid screening and discovering genetic variations.

Colorectal cancer is a cancer that starts in the colon or the rectum. The American Cancer Society estimate that about 4.8% men and 4.3% women in the United States will develop colorectal cancer during their lifetime. It is one of the most leading causes of cancer death worldwide. Atila Colorectal Cancer Panel NGS library preparation Kit targets 149 amplicon regions of 21 Colorectal cancer related genes, including AKT1, APC, AXIN1, BRAF, CTNNB1, EP300, FBXW7, KRAS, MAP2K1, MAP2K4, MDM2, MLH1, MSH2, MSH6, MUTYH, PIK3CA, PIK3R1, PMS1, PMS2, TCF7L2, and TP53 etc. The resulting sample library is compatible with Illumina NGS sequencing. The current method employs novel primer design strategies, greatly simplifies sample preparation procedures, and enables rapid screening and discovering genetic variations.

Special Features:

  • High sensitivity: The kit can use as low as 100pg purified DNA;
  • Short preparation time: Sample library is ready within 2.5-3 hours.
  • Simple procedures: Hands-on time is as short as 30 minutes.
  • High specificity: high on target rate and high uniformity.

Technical Specs:

  • Sample Types: purified DNA, cell free DNA, FFPE DNA
  • Sensitivity: 100pg-1ng purified DNA, 1ng-10ng cell free DNA or FFPE DNA
  • Assay Time: 2.5-3 hours
  • Hands-On Time: 30 minutes
  • System Compatibility: MiniSeq, MiSeq
  • Method: Multiplex PCR, targeted amplicon sequencing

* Order Information:

Cat. No. Description Size
ACPN-HT-100 Hotspot Cancel Panel NGS library preparation Kit 100 reactions
ACPN-BR-100 Breast Cancer Panel 100 reactions
ACPN-LN-100 Lung Cancer Panel 100 reactions
ACPN-CO-100 Colorectal Cancer Panel 100 reactions

*  These products are for research use only in US

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