Clostridium difficile PCR Detection Kit
This kit is designed for rapid qualitative detection of C. difficile DNA isolated from patient stool samples.
Each kit includes 100 reactions.
This product is for research use only.
Clostridium difficile PCR Detection Kit
The Clostridium difficile PCR Detection Kit provides a PCR master mix which contains reagents and enzymes for PCR amplification and a primer mix which are specific to the C. difficile genome. In addition, the kit provides an internal control to identify possible PCR inhibition or sample preparation failure.
Clostridium difficile (C. difficile) is a species of spore-forming bacteria that produces multiple toxins and causes Clostridium difficile colitis, shown as diarrhea, bloating, and abdominal pain. It is a major cause of Healthcare-associated infections (HAIs) due to its antibiotic resistance and tolerance to extreme conditions in spore form.
Features Include
Technical Specs
Workflow Overview for the Clostridium difficile PCR Detection Kit
This section is for demonstrative purposes only and may be incomplete or inaccurate. Always refer to the product instructions for precise guidelines and directions.
Prepare the PCR reaction mix by combining 12.5μL x (N+2) of PCR master mix with 5μL x (N+2) of primer mix.
Finalize by adding H2O 5.5μL x (N+2) to complete the reaction mix.
Add 23μL of reaction mix per well along with 2μL of extracted DNA and internal control.
Perform PCR.
